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transplex ® complete whole transcriptome amplification kit  (Millipore)


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    Structured Review

    Millipore transplex ® complete whole transcriptome amplification kit
    Transplex ® Complete Whole Transcriptome Amplification Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transplex+%C2%AE+complete+whole+transcriptome+amplification+kit/wta2+kit/pmc11356395-63-6-13
    Average 90 stars, based on 1 article reviews
    transplex ® complete whole transcriptome amplification kit - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Generated:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.

    Amplification:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.

    Microscopy:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.

    Software:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.

    Quantitative RT-PCR:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.

    Purification:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.

    Polymerase Chain Reaction:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.

    Labeling:

    Article Title: Oxidative Stress Is Associated with Overgrowth in Drosophila l(3)mbt Mutant Imaginal Discs
    Article Snippet: Briefly, cDNA was generated from 25 ng of RNA using the TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and 17 cycles of amplification.

    Article Title: Fast-Proliferating Adipose Tissue Mesenchymal-Stromal-Like Cells for Therapy
    Article Snippet: Briefly, 25 ng of total RNA was amplified using the TransPlex ® Complete Whole Transcriptome Amplification kit (Sigma) and subsequently labeled using GeneChip Mapping 10K Xba Assay kit (Affymetrix), according to manufacturer's instructions.

    Article Title: Tumoral Immune Suppression by Macrophages Expressing Fibroblast Activation Protein-Alpha and Heme Oxygenase-1
    Article Snippet: Images were acquired using a Leica SP2 confocal microscope and associated LCS analysis software. qRT PCR analysis mRNA from tumor tissue and sorted cells was extracted using the Absolutely RNA ® Miniprep Kit (Agilent Technologies). mRNA from sorted populations was amplified using the Transplex ® Complete whole transcriptome amplification kit (Sigma-Aldrich), and subsequent cDNA purified using a PCR Purification Kit (Qiagen) according to the manufacturer’s protocols. qRT PCR was performed as previously described using the SuperScript® III Platinum ® One-step qRT-PCR system (Invitrogen) ( 8 ).

    Article Title: Diverse RNA viruses discovered in multiple seagrass species
    Article Snippet: Transcriptome libraries were prepared using a TransPlex ® Complete Whole Transcriptome Amplification Kit (Sigma-Aldrich TM , USA) in accordance with the recommended protocol.



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    Millipore transplex® complete whole transcriptome amplification kit (wta2
    Whole <t>transcriptome</t> analysis was performed on the left ventricle (LV), right ventricle (RV), left atrium (LA) and the right atrium (RA) of rhesus monkey (Macaca mulatta) fetuses that were exposed maternally to a 400 µg/kg body weight, Bisphenol A (BPA), relative to matched control fetuses, either during, (A) early gestation, EG (days; 50–100±2) or, (B) late gestation, LG (days 100±2–term). Genes that changed by greater than ±1 log 2 fold change (1 LFC = 2 fold change) at an unadjusted p≤0.01 were considered significant and differentially expressed. Bars represent the number of upregulated (positive y-axis) or downregulated (negative y-axis) genes in each of the tissues.
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    Image Search Results


    Whole transcriptome analysis was performed on the left ventricle (LV), right ventricle (RV), left atrium (LA) and the right atrium (RA) of rhesus monkey (Macaca mulatta) fetuses that were exposed maternally to a 400 µg/kg body weight, Bisphenol A (BPA), relative to matched control fetuses, either during, (A) early gestation, EG (days; 50–100±2) or, (B) late gestation, LG (days 100±2–term). Genes that changed by greater than ±1 log 2 fold change (1 LFC = 2 fold change) at an unadjusted p≤0.01 were considered significant and differentially expressed. Bars represent the number of upregulated (positive y-axis) or downregulated (negative y-axis) genes in each of the tissues.

    Journal: PLoS ONE

    Article Title: Maternal Bisphenol A Exposure Impacts the Fetal Heart Transcriptome

    doi: 10.1371/journal.pone.0089096

    Figure Lengend Snippet: Whole transcriptome analysis was performed on the left ventricle (LV), right ventricle (RV), left atrium (LA) and the right atrium (RA) of rhesus monkey (Macaca mulatta) fetuses that were exposed maternally to a 400 µg/kg body weight, Bisphenol A (BPA), relative to matched control fetuses, either during, (A) early gestation, EG (days; 50–100±2) or, (B) late gestation, LG (days 100±2–term). Genes that changed by greater than ±1 log 2 fold change (1 LFC = 2 fold change) at an unadjusted p≤0.01 were considered significant and differentially expressed. Bars represent the number of upregulated (positive y-axis) or downregulated (negative y-axis) genes in each of the tissues.

    Article Snippet: Due to the limitations in the quantity of tissue available from the LA and RA, cDNA preparations from both these tissue were prepared by employing a PCR based amplification approach available through TransPlex® Complete Whole Transcriptome Amplification Kit (WTA2) (Sigma, St. Louis, MO, USA).

    Techniques: